When it comes to treating large cartilage defects, allogenic osteochondral tissue (OCT) is often used. However, the gradual reduction in cell viability during cold preservation can negatively affect transplantation outcomes. In order to increase the availability of high-quality OCTs and improve treatment outcomes, researchers have been searching for ways to improve the storage methods that maintain the cell viability of OCTs.
Recently, a study was conducted to evaluate whether long-term hydrogen delivery to preservation solution improved the viability of rat OCTs during cold preservation. The results were promising. Hydrogen-supplemented Dulbecco’s Modified Eagles Medium (DMEM) and University of Wisconsin (UW) solution both significantly improved the cell viability of OCTs during preservation at 4°C for 21 days compared to non-supplemented media. However, the long-term cold preservation of OCTs in DMEM containing hydrogen was associated with the most optimal maintenance of chondrocytes with respect to viability and morphology.
This is exciting news for those suffering from large cartilage defects, as it suggests that OCTs preserved in DMEM supplemented with hydrogen could be a promising material for the repair of these defects in the clinical setting.
It is important to note that this study was conducted on rat OCTs and more research is needed to determine the effectiveness and safety of using hydrogen-supplemented preservation solution in human cartilage repair. As always, please consult with a qualified healthcare professional before making any decisions about your treatment.
Medical advice disclaimer: The content of this blog post is not intended to be a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of your physician or other qualified healthcare provider with any questions you may have regarding a medical condition.
Study: https://pubmed.ncbi.nlm.nih.gov/25506061/
Yamada, T., et al., Hydrogen supplementation of preservation solution improves viability of osteochondral grafts. ScientificWorldJournal, 2014. 2014: p. 109876.
